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plcγ1 antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology plcγ1 antibody
    Plcγ1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plcγ1 antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 310 article reviews
    plcγ1 antibody - by Bioz Stars, 2026-02
    94/100 stars

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    Samples prepared using ProtiFi S-Trap show superior identification of biologically relevant, T cell receptor responsive pY sites. (A) Simplified schematic of T cell receptor signalling. (B-E) Volcano plot analysis comparing pY site abundance between 2 minutes and 0 minutes of stimulation for samples prepared using Waters Sep-Pak C18 columns, Pierce C18 spin columns, TECAN WWP2 NBE columns, and ProtiFi S-Trap spin columns, respectively. Values to the left indicate the number of unique pY sites in a given threshold (0.1 > q ≥ 0.05, 0.05 > q ≥ 0.01, 0.01 > q from bottom to top). (F) Western blot analysis of <t>PLCγ1</t> <t>Y783</t> with accompanying quantification. *** indicates p < 0.001 by a Welch’s T-test. (G) Heatmaps comparing 2 minutes and 0 minutes of T cell receptor stimulation for unique pY sites. Grey squares indicate n ≤ 3 replicates for a given pY site in either 2- or 0-minute groups. * indicates 0.1 > q ≥ 0.05, ** indicates 0.05 > q ≥ 0.01, *** indicates 0.01 > q.
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    Image Search Results


    Samples prepared using ProtiFi S-Trap show superior identification of biologically relevant, T cell receptor responsive pY sites. (A) Simplified schematic of T cell receptor signalling. (B-E) Volcano plot analysis comparing pY site abundance between 2 minutes and 0 minutes of stimulation for samples prepared using Waters Sep-Pak C18 columns, Pierce C18 spin columns, TECAN WWP2 NBE columns, and ProtiFi S-Trap spin columns, respectively. Values to the left indicate the number of unique pY sites in a given threshold (0.1 > q ≥ 0.05, 0.05 > q ≥ 0.01, 0.01 > q from bottom to top). (F) Western blot analysis of PLCγ1 Y783 with accompanying quantification. *** indicates p < 0.001 by a Welch’s T-test. (G) Heatmaps comparing 2 minutes and 0 minutes of T cell receptor stimulation for unique pY sites. Grey squares indicate n ≤ 3 replicates for a given pY site in either 2- or 0-minute groups. * indicates 0.1 > q ≥ 0.05, ** indicates 0.05 > q ≥ 0.01, *** indicates 0.01 > q.

    Journal: bioRxiv

    Article Title: Evaluating First-Pass, High Protein Capacity Desalting Techniques For Phosphoproteomics Applications

    doi: 10.1101/2025.06.03.657744

    Figure Lengend Snippet: Samples prepared using ProtiFi S-Trap show superior identification of biologically relevant, T cell receptor responsive pY sites. (A) Simplified schematic of T cell receptor signalling. (B-E) Volcano plot analysis comparing pY site abundance between 2 minutes and 0 minutes of stimulation for samples prepared using Waters Sep-Pak C18 columns, Pierce C18 spin columns, TECAN WWP2 NBE columns, and ProtiFi S-Trap spin columns, respectively. Values to the left indicate the number of unique pY sites in a given threshold (0.1 > q ≥ 0.05, 0.05 > q ≥ 0.01, 0.01 > q from bottom to top). (F) Western blot analysis of PLCγ1 Y783 with accompanying quantification. *** indicates p < 0.001 by a Welch’s T-test. (G) Heatmaps comparing 2 minutes and 0 minutes of T cell receptor stimulation for unique pY sites. Grey squares indicate n ≤ 3 replicates for a given pY site in either 2- or 0-minute groups. * indicates 0.1 > q ≥ 0.05, ** indicates 0.05 > q ≥ 0.01, *** indicates 0.01 > q.

    Article Snippet: Primary antibodies: pY clone 4G10 (1:2000, Cell Signalling Technologies #96215), GAPDH (1:10000, Millipore Sigma #G9545), PLCγ1 (1:10000, Millipore Sigma #05-163), PLCγ1 Y783 (1:2000, Cell Signalling Technologies #2821), Erk1/2 (Cell Signalling Technologies #9107), Erk T202Y204 (1:2000, Cell Signalling Technologies #9101) Secondary antibodies: IRDye 680RD Goat anti-Mouse IgG (LI-COR #926-68070) IRDye 800CW Goat anti-Mouse IgG (LI-COR #926-33210), IRDye 680RD Donkey anti Rabbit IgG (LI-COR #926-68073), IRDye 800CW Donkey anti Rabbit IgG (LI-COR #926-32213)

    Techniques: Western Blot